The process of cellular mitosis is captured with fluorescence microscopy utilizing A549 cells in monolayer culture triple-stained with fluorophores containing TRITC, FITC, and DAPI. Presented below is a photomicrograph of the cells illustrating nuclei and chromosomes, myosin, and vinculin.

Incident light fluorescence microscopy is growing rapidly in importance as an investigational tool in the fields of medical and biological research. All photomicrographs in this gallery were taken with Olympus microscopes employing UIS optics and a PM-30 automatic camera system.

The specimen is a monolayer of A549 human lung carcinoma in tissue culture that was triple-stained using a mixture of TRITC, FITC, and DAPI. Photomicrographs were recorded utilizing a UPL 40x apochromatic objective coupled to a WIBA/WU dichroic filter combination. A 3.3x projection photo eyepiece was used to transfer light from the intermediate image plane to the photographic emulsion.

Originally cultivated from human lung carcinoma, the cell line A549 is being used to shed light upon the secrets of certain respiratory ailments. A594 continues to provide investigational scientists with insights into research areas as diverse as tuberculosis interaction, asthma-inducing viral infections (including the rhinovirus), asbestos-related tissue damage, and smoking-related emphysema.