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Pollen is a seasonal problem for millions of people around the world who suffer from allergenic reactions to the antigens embedded on the outer casing of these microscopic grains. The image presented above reveals green autofluorescence of tiny pollen grains visualized utilizing widefield fluorescence microscopy. Photomicrographs were recorded utilizing a UPL 10x fluorite objective coupled to a WBV dichroic filter combination. A 3.3x projection photo eyepiece was used to transfer light from the intermediate image plane to the photographic emulsion. |