Interactive Tutorials
Virtual Microscopy
Movie Gallery
Downloads
Galleries
Microscopy Primer
Light and Color
Basic Concepts
Special Techniques
Fluorescence
Confocal Microscopy
Digital Imaging
Photomicrography
Web Resources
MIC-D Microscope
Resource Center

Interactive Java Tutorials

Reflected Light Fluorescence Microscopy

Instructions and a discussion about how to operate this tutorial appear immediately following the Java applet window.

Interactive Java Tutorial
ATTENTION
Our servers have detected that your web browser does not have the Java Virtual Machine installed or it is not functioning properly. Please install this software in order to view our interactive Java tutorials. Visitors using the Netscape and Microsoft Internet Explorer browsers can download the appropriate software from the websites where the browsers are distributed. Please do not contact us for information about specific URLs where this software can be obtained. 

This tutorial explores the light path and filtration in reflected fluorescence microscopy. The sliders and inside/outside button control the tutorial operation. Microscope diaphragms are operated by the Aperture Diaphragm and Field Diaphragm sliders, which allow control over the iris apertures located in the episcopic illuminator. A fluorescence cube is located in the head of the microscope and has a slider that controls the filter excitation and emission wavelengths. Adjusting this slider produces corresponding changes in the wavelength of excitation and emission light, and these changes reflect new colors of filtered light in the optical pathways. The outside/inside button toggles between external and cut-away internal views of the microscope.

Contributing Authors

Mortimer Abramowitz - Olympus America, Inc., Two Corporate Center Drive., Melville, New York, 11747.

Matthew J. Parry-Hill, John C. Long, and Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.


BACK TO SPECIALIZED MICROSCOPY TECHNIQUES

.  
. Copyright 2000-2003 Olympus America, Inc. . . .
.